Part:BBa_K2957006

CMV, IL-8 (CXCL-8), Flag Tag

Contents: CMV, IL-8 (CXCL-8), Flag Tag, SynthpA, with Carb resistance

What is it?: A Composite part; it is a genetic circuit with CMV promoter and IL-8 gene allowing for constitutive IL-8 expression

What does it do?: It's responsible for the expression of the chemokine, IL-8 (CXCL8) with Flag tag. It was important for our project in particular because of its involvement in the immune system and is known to create neutrophil swarming.

How to use it?: This part was created with Type IIS Cloning and transfected into HEKs. It also has a Flag tag attached to the IL8 so this will allow it to be tracked and then blotted with ELISA. See more on the Parts Overview Page or MIT iGEM 2019 wiki: <a href=https://2019.igem.org/Team:MIT/Parts>."

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal XbaI site found at 1137
  Illegal SpeI site found at 92
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal SpeI site found at 92
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 1031
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal XbaI site found at 1137
  Illegal SpeI site found at 92
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal XbaI site found at 1137
  Illegal SpeI site found at 92
  Illegal AgeI site found at 1143
  
• 1000
  COMPATIBLE WITH RFC[1000]